|ELISA (ELISA)||0.1-0.25 µg/mL (coating)|
|Western Blot (WB)||0.02 µg/mL|
|ELISA (ELISA)||See 3 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Pig|
|Published species reactivity||Pig , Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant porcine TNF alpha (Baculovirus-derived)|
MP390 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa. This antibody will not cross-react with human or mouse TNFa, mouse TNFb, or porcine TNFb.
This is a carrier-free low endotoxin formulation, <1.0 EU/mg.
This product can be used as a coating antibody in a sandwich ELISA (100 µg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product # M302B, 0.1-0.65 µg/mL). For Western blot, this antibody has been successfully used in conjunction with 0.02 µg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product # 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFa (Product # RPTNFAI), followed by visualization using SuperSignal® West Pico Chemiluminescent Substrate (Product # 34080).
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cachectin; TNF-a; TNF-alpha; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor-necrosis factor
Gene Aliases: SBAB-548A10.2; TNF; TNFA; TNFSF2
UniProt ID: (Pig) P23563
Entrez Gene ID: (Pig) 397086
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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