|Western Blot (WB)||Assay Dependent|
|ELISA (ELISA)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human TNF alpha from Pichia pastoris.|
|Storage buffer||PBS with 5% trehalose|
MTNFAI targets TNF Alpha in ELISA, and Western blot applications and shows reactivity with Human samples. The MTNFAI antibody (clone E10043) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M3TNFABI (biotinylated conjugate of clone E10041).
The MTNFAI immunogen is recombinant human TNF alpha from Pichia pastoris.
MTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Protein Aliases: APC1 protein; Cachectin; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; Tumor necrosis factor; tumor necrosis factor alpha; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Gene Aliases: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F
UniProt ID: (Human) P01375
Entrez Gene ID: (Human) 7124