|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||Assay-dependent|
|ELISA (ELISA)||See 2 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant, murine TNF alpha expressed in E. coli.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, do not freeze|
MA1-20303 detects TNF alpha in mouse and rat samples. Reactivity with mouse TNF beta has not been tested.
MA1-20303 has been successfully used in ELISA procedures.
The MA1-20303 immunogen is recombinant, mouse TNF alpha expressed in E. coli.
Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cachectin; DIF; MGC151434; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Gene Aliases: DIF; RATTNF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2
UniProt ID: (Mouse) P06804
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