|Flow Cytometry (Flow)||0.4 µg|
|Immunofluorescence (IF)||1:20 - 1:100|
|Western Blot (WB)||1:500 - 1:2000|
|Immunocytochemistry (ICC)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Human embryonal carcinoma cell line 2102Ep.|
|Purification||Affinity chromatography - MBP|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-023 has been successfully used in flow cytometry, immunofluorescence and Western blot applications on human samples.
TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Keratan sulfate antigen TRA1-60; TRA160
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