|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||3-5 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 410-460 of human TRAF6.|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: human thymus, testis cell lysate, antigen standard for TRAF6 (transient overexpression lysate).
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; MGC:3310; RING finger protein 85; RING-type E3 ubiquitin transferase TRAF6; RNF85; TNF receptor-associated factor 6; TNF receptor-associated factor 6, E3 ubiquitin protein ligase
Gene Aliases: 2310003F17Rik; AI851288; C630032O20Rik; MGC:3310; RNF85; TRAF6
Molecular Function: signaling molecule
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