|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide corresponding to residues 17-35 of human TRAIL.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This peptide sequence has been shown to be involved in TRaIL and DR5 (TRaIL-R2) interaction.
Suggested positive control: Jurkat whole cell lysate.
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand) (6,7). TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL3-6. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.
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Protein Aliases: Apo-2 Ligand; Apo-2L; Apo2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Protein TRAIL; superfamily member 10; TNF-related apoptosis inducing ligand TRAIL; TNF-Related Apoptosis-Inducing Ligand; TNFSF10; TRAIL protein; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; Tumor necrosis factor ligand; tumor necrosis factor ligand 6A; Tumor necrosis factor ligand superfamily member 10
Gene Aliases: Apo-2L; APO2L; CD253; TL2; TNFSF10; TNLG6A; TRAIL
UniProt ID: (Human) P50591
Entrez Gene ID: (Human) 8743
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