|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to 15 residues near the N-terminus of mouse TRIAD3A.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is testis or peripheral blood leukocytes.
Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2810055G22Rik; AI647468; AU019462; C86502; E3 ubiquitin ligase; E3 ubiquitin-protein ligase RNF216; F830018F18Rik; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; Triad domain-containing protein 3; TRIAD3; Ubce7ip1; UbcM4-interacting protein 83; Ubiquitin-conjugating enzyme 7-interacting protein 1; UIP83
Gene Aliases: 2810055G22Rik; AI647468; AU019462; C86502; F830018F18Rik; Rnf216; TRIAD3; Ubce7ip1; UIP83; Zin
UniProt ID: (Mouse) P58283
Entrez Gene ID: (Mouse) 108086
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.