|Immunocytochemistry (ICC)||10 ug/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunohistochemistry (IHC)||1 µg/ml|
|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~25kDa. A suggested positive control is mouse brain tissue lysate.
PA5-20048 can be used with blocking peptide PEP-0167.
TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5-a variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1 , albeit at differing efficiencies. The TRIM5δ isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5δ also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: RING finger protein 88; RING-type E3 ubiquitin transferase TRIM5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa; tripartite motif protein TRIM5; Tripartite motif-containing protein 5
Gene Aliases: RNF88; TRIM5; TRIM5alpha
UniProt ID: (Human) Q9C035
Entrez Gene ID: (Human) 85363
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