|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Hamster, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG1, k|
|Immunogen||Residues 167-374 of TSG101, expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-23296 detects TSG101 in human, mouse, rat, hamster, and monkey samples.
MA1-23296 has been successfully used in ELISA, FACS, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation and Western blot procedures. Suggested positive controls for this product are MOLT4 and HeLa.
The MA1-23296 immunogen corresponds to residues 167-374 of TSG101, expressed in E. coli.
Store at 4°C short term. For long term storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a luecine-zipper motif within a coiled-coil domain near the carboxyl terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ESCRT-I complex subunit TSG101; TSG10; Tumor supressor; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein; VPS23
Gene Aliases: AI255943; CC2; Rw; TSG10; TSG101; VPS23
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