|Immunocytochemistry (ICC)||10 ug/ml|
|Western Blot (WB)||0.5 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide near the amino terminus of human TYW2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||4° C|
A suggested positive control is K562 cell lysate.
PA5-21153 can be used with blocking peptide PEP-1267.
TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha-amino-alpha-carboxypropyl transferase TYW2; homolog of yeast tRNA methyltransferase; TRM12, TYW2; tRNA methyltranferase 12 homolog; tRNA methyltransferase 12; tRNA wybutosine-synthesizing protein 2 homolog; tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase; tRNA-yW synthesizing protein 2; tRNA-yW-synthesizing protein 2
Gene Aliases: 4632406N01Rik; AI449275; TRM12; TRMT12; TYW2
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