|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to a region of human Tankyrase within residues 250-350. The actual peptide sequence is proprietary.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
In Western blot, this antibody recognizes an endogenous band at ~150 kDa in whole cell extract and a band at ~180 kDa in baculovirus-derived his-tagged protein.
Suggested positive control: Hela whole cell extract.
The ~142 kDa Tankyrase protein is ubiquitously expressed in mammalian cells and is localized to human telomeres. It has homology to ankyrins and to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase binds to TRF1 (telomeric repeat binding factor-1), which negatively regulates the maintenance of telomere length.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADP-ribosyltransferase diphtheria toxin-like 5; ARTD5; PARP-5a; PARP5A; PARPL; pART5; Poly [ADP-ribose] polymerase 5A; TANK1; Tankyrase I; tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase; Tankyrase-1; TIN1; TINF1; TNKS-1; TNKS1; TRF1-interacting ankyrin-related ADP-ribose polymerase; TRF1-interacting ankyrin-related ADP-ribose polymerase 1
Gene Aliases: ARTD5; PARP-5a; PARP5A; PARPL; pART5; TIN1; TINF1; TNKS; TNKS1
UniProt ID: (Human) O95271
Entrez Gene ID: (Human) 8658
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.