|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||1-2 ul|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant, human thioredoxin reductase 1 protein expressed in E. coli.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.01% BSA, 50% glycerol, 0.15M NaCl|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is HeLa cells.
The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxidoreductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence (-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; KM-102-derived reductase-like factor; oxidoreductase; testis tissue sperm-binding protein Li 46a; Thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; Thioredoxin reductase TR1; TR; trxR1
Gene Aliases: GRIM-12; GRIM12; KDRF; TR; TR1; TRXR1; TXNR; TXNRD1
UniProt ID: (Human) Q16881
Entrez Gene ID: (Human) 7296
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