|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (IHC)||See 9 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human TS enzyme|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13512 targets Thymidylate Synthase in ELISA, FACS, IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-13512 immunogen is recombinant human TS enzyme.
Thymidylate Synthase (TS) (EC 184.108.40.206) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Thymidylate synthase; TS; TSase
Gene Aliases: HST422; OK/SW-cl.29; TMS; TS; TYMS
UniProt ID: (Human) P04818
Entrez Gene ID: (Human) 7298
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.