|Flow Cytometry (Flow)||1:100-1:1000 (10 µl for 10^6 cells in 100 µl)|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1/1000|
|Immunocytochemistry (ICC)||See 3 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide: External domain of human TSH receptor linked to GST|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-23149 detects TSH Receptor in human samples.
MA1-23149 has been successfully used in FACS, immunohistochemistry (frozen and paraffin), immunoprecipitation and Western Blot procedures.
MA1-23149 immunogen corresponds to Synthetic peptide: External domain of human TSH receptor linked to GST.
The TSH receptor, a G protein coupled seven transmembrane receptor, is present on the basal surface of thyroid follicular cells. It is involved in regulating thyrocyte cell growth and function by mediating thyroid stimulating hormone (TSH) action. The TSH receptor is also the target autoantigen in autoimmune thyroid diseases. Autoantibodies to the TSH receptor that stimulate cAMP production in thyrocyte cells, called thyroid stimulating antibodies, are responsible for the hyperthyroidism of Graves' Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2; Thyroid-stimulating hormone receptor; Thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH Receptor; TSH-R
Gene Aliases: CHNG1; hTSHR-I; LGR3; TSHR
UniProt ID: (Human) P16473
Entrez Gene ID: (Human) 7253
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