|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||5-10 µg/mL|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide, corresponding to aa 1513-1530 (RAKKPIKYLEESDEDDLF) from the C-terminus of human topoisomerase IIalpha|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
PA5-16322 targets Topoisomerase II alpha in IF, IHC, IP, and WB applications and shows reactivity with Human samples.
The PA5-16322 immunogen is a synthetic peptide, corresponding to aa 1513-1530 (RAKKPIKYLEESDEDDLF) from the C-terminus of human topoisomerase IIalpha.
Topo IIalpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo IIalpha is also implicated in drug resistance of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 170 kD; alpha isozyme; DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; OTTHUMP00000207108; topoisomerase (DNA) II alpha 170kDa
Gene Aliases: TOP2; TOP2A; TP2A
UniProt ID: (Human) P11388
Entrez Gene ID: (Human) 7153
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