|ELISA (ELISA)||5-10 µg/mL|
|Tested Species reactivity||Chemical|
|Host / Isotype||Sheep / IgG|
|Contains||0.09% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-75471 specifically detects Tramadol.
PA1-75471 has been successfully used in ELISA applications.
The PA1-75471 immunogen is Tramadol(N)-BTG.
Tramadol is only found in individuals that have used or taken this drug. It is a narcotic analgesic proposed for moderate to severe pain. It may be habituating. [PubChem] Tramadol and its O-desmethyl metabolite (M1) are selective, weak OP3-receptor agonists. Opiate receptors are coupled with G-protein receptors and function as both positive and negative regulators of synaptic transmission via G-proteins that activate effector proteins. As the effector system is adenylate cyclase and cAMP located at the inner surface of the plasma membrane, opioids decrease intracellular cAMP by inhibiting adenylate cyclase. Subsequently, the release of nociceptive neurotransmitters such as substance P, GABA, dopamine, acetylcholine and noradrenaline is inhibited. The analgesic properties of Tramadol can be attributed to norepinephrine and serotonin reuptake blockade in the CNS, which inhibits pain transmission in the spinal cord. The (+) enantiomer has higher affinity for the OP3 receptor and preferentially inhibits serotonin uptake and enhances serotonin release. The (-) enantiomer preferentially inhibits norepinephrine reuptake by stimulating alpha(2)-adrenergic receptors. [National Center for Biotechnology Information. PubChem Compound Database; CID=33741]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C16H25NO2; Ralivia; Tramal; Ultram
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