|Flow Cytometry (Flow)||1/20 - 1/50|
|Immunofluorescence (IF)||1:50 - 1:200|
|Western Blot (WB)||1:500|
|Immunohistochemistry (IHC)||See 5 publications below|
|Immunocytochemistry (ICC)||See 23 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 4 publications below|
|Tested Species reactivity||Human, Non-human primate, Rat|
|Published species reactivity||Rat , Non-human primate , Hamster , Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues L(1) P S A S K P N N T S S E N N P P(17) C of rat TGN38.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-063 detects trans-Golgi network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
MA3-063 has been successfully used in Western blot, immunofluorescence, immunocytochemistry and FACS procedures. By Western blot, this antibody detects an ~38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal Western blot conditions, this antibody detects an 85-95 kDa band depending on cell type. Immunofluorescence staining of TGN38 in NRK cells with MA3-063 results in staining predominantly in the TGN.
In immunofluorescence, it has been shown that methanol works best as a fixative.
The MA3-063 immunogen is a synthetic peptide corresponding to residues L(1) P S A S K P N N T S S E N N P P(17) C of rat TGN38.
The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways.
TGN38 and TGN41, an isoform of the former, are integral membrane proteins which are predominantly localized to the TGN in NRK cells but which can also be observed at the cell surface. Complexes which include TGN38, low molecular weight G proteins and p62 are required for the formation of TGN-derived vesicles. This requirement has lead people to believe that TGN38 is important in the trafficking of proteins from the TGN to the cell surface. Brefeldin A causes the contents of the Golgi stack to be redistributed into the endoplasmic reticulum while the TGN collapse upon the microtubule organizing center. This makes immunofluorescence detection of TGN38 following brefeldin A treatment a useful tool for the discrimination of the TGN from the Golgi stack.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: TGN38; TGN38 homolog; TGN46; TGN48; Trans-Golgi network integral membrane protein 2; trans-Golgi network protein (46, 48, 51kD isoforms); Trans-Golgi network protein TGN51
Gene Aliases: TGN38; TGN46; TGN48; TGN51; TGOLN2; TTGN2
UniProt ID: (Human) O43493
Entrez Gene ID: (Human) 10618
Molecular Function: membrane traffic protein
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