|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 7 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Mouse, Rabbit|
|Published species reactivity||Rabbit Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rabbit skeletal muscle triads.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-931 detects triadin from mouse and rabbit skeletal muscle tissues. This antibody does not detect triadin from cardiac tissues.
MA3-931 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a single ~95 kDa band representing triadin in rabbit skeletal muscle extracts. Immunofluorescence staining of triadin in rabbit skeletal muscle with MA3-931 results in its co-localization with ryanodine receptor, which is consistent with the association of ryanodine receptor and triadin in triads.
The MA3-931 antigen is rabbit skeletal muscle triads.
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR and quote;s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR and quote;s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR and quote;s and RyR and quote;s.
Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cardiac triadin; junctional sarcoplasmic reticulum glycoprotein; TRDN; Triadin; TRISK
Gene Aliases: 2310045H21Rik; EG432451; TDN; TRDN
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