|Western Blot (WB)||1:1000|
|Tested Species reactivity||Luciferase|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant TurboLuc luciferase expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-178 detects an ~16-18 kDa protein in Tluc expressing cells.
PA1-178 can be used for activity-independent verification of Tluc expression in qIP assays.
TurboLuc™ (Tluc) is a novel intracellular luciferase derived from the marine copepod Metridia luciferase family. The Tluc gene was developed using directed mutagenesis for optimal performance and codon optimized for mammalian cell expression. Tluc is an ATP-independent enzyme that utilizes the substrate coelenterazine to produce intense bioluminescence light. Tluc is smaller and brighter than other commonly used luciferases, including firefly and Renilla. The intense bioluminescence greatly enhances sensitivity of Tluc luciferase-based assays, enabling detection of very minute amounts of luciferase (signal) in very sensitive assays such as protein interaction qIP assays (Prod # 82032, 82033, 82035, 82036).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: TurboLuc luciferase synthetic
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