|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 51 and 516 of Human UHRF1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27969 targets UHRF1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-27969 immunogen is recombinant fragment corresponding to a region within amino acids 51 and 516 of Human UHRF1.
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E3 ubiquitin-protein ligase UHRF1; hNP95; hUHRF1; HuNp95; ICBP90; Inverted CCAAT box-binding protein of 90 kDa; Np95; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; RNF106; Transcription factor ICBP90; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; Ubiquitin-like-containing PHD and RING finger domains protein 1
Gene Aliases: hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1
UniProt ID: (Human) Q96T88
Entrez Gene ID: (Human) 29128
Molecular Function: ubiquitin-protein ligase
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.