|ChIP assay (ChIP)||1:20-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human UHRF1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 90 and 110 kDa.
Purity is >95% by SDS-PAGE.
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5-CCAAT-3 box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1s ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E3 ubiquitin-protein ligase UHRF1; hNp95; hUHRF1; HuNp95; ICBP90; Inverted CCAAT box-binding protein of 90 kDa; NP95; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; RNF106; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; Ubiquitin-like-containing PHD and RING finger domains protein 1
Gene Aliases: hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1
UniProt ID: (Human) Q96T88
Entrez Gene ID: (Human) 29128
Molecular Function: ubiquitin-protein ligase
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