|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 327-346 of mouse USF-2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of 43kDa (USF-1) and 44kDa (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHb12; c-fos interacting protein; Class B basic helix-loop-helix protein 12; FIP; FOS-interacting protein; Major late transcription factor 2; Upstream stimulatory factor 2; Upstream transcription factor 2
Gene Aliases: BHLHB12; FIP; USF2
UniProt ID: (Human) Q15853
Entrez Gene ID: (Human) 7392
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