|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1.0-2 µg/ml|
|Tested Species reactivity||Dog, Chicken, Horse, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (45 NVALSDGHNTAEEDMEDDT 63) of USP7 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
USP7 (ubiquitin specific peptidase 7) is a nuclear protein belonging to the peptidase C19 family with a MATH domain and is known to cleave ubiquitin fusion protein substrates. It is a monomer that interacts with proteins like TP53, MDM2 and UBXN6 and also with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1 and this binding may modulate the substrate specificity or activity of USP7 to stabilize viral proteins. USP7 deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and is known to induce TP53-dependent cell growth repression and apoptosis. USP7 is generally inhibited by N-ethyl-maleimide (NEM) and divalent cations. Though it tolerates high concentrations of NaCl, it is inhibited at concentrations of 195 mM and higher. USP7 is widely expressed in most of the tissues and might be present in a minority of ND10 nuclear bodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Deubiquitinating enzyme 7; HAUSP; Herpes virus-associated ubiquitin-specific protease; herpes-virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; mHAUSP; rHAUSP; TEF1; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific protease 7; ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; ubiquitin thiolesterase 7; Ubiquitin-specific-processing protease 7
Gene Aliases: 2210010O09Rik; AA409944; AA617399; AU019296; AW548146; C80752; HAUSP; TEF1; UBP; USP7
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