|Immunoprecipitation (IP)||1-2 µl|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide, KLH coupled, corresponding to the C-terminal residues of human Ubiquitin +1 protein.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is Bosc23 cells transfected with GFP-Ub+1.
Ubiquitin+1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially ub+1 is accumulated in aggregates containing amyloid-beta and phosphorylated-tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer's disease. Also ub +1 acts as an aggravating factor in polyglutamine-induced neurodegeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: epididymis secretory protein Li 50; MGC127041; polyubiquitin B; Polyubiquitin-B; RPS27A; UBB; UBC
Gene Aliases: HEL-S-50; UBB
UniProt ID: (Human) P0CG47
Entrez Gene ID: (Human) 7314
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