|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||5 µl|
|Western Blot (WB)||1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant, human ubiquitin GST-fusion protein expressed in E. coli.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is Bosc23 cells transfected with GFP-Ub.
Ubiquitin (Ub) is a small protein that is composed of 76 amino acids. Ub is found only in eukaryotic organisms and is highly conserved. Ub can exist either in free form or as part of a complex with other proteins. In the latter case, Ub is attached (conjugated) to proteins through a covalent bond between the glycine at the C-terminal end of Ub and the side chains on the lysine proteins; Ubiquitination.-Ub functions to regulate protein turnover in a cell by closely regulating the degredation of specific proteins. Ubiquitin has been immunohistochemically localized to a number of pathological inclusions, including ; Lewy bodies of Parkinson's disease, neurofibrillary of Alzheimer's disease, Pick bodies of Pick's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 60S ribosomal protein L40; epididymis secretory protein Li 50; HUBCEP52; polyubiquitin B; Polyubiquitin-B; RPL40; Ubiquiti; Ubiquitin 52 amino acid fusion protein; Ubiquitin A52 residue ribosomal protein fusion product 1
Gene Aliases: HEL-S-50; UBB
UniProt ID: (Human) P0CG47
Entrez Gene ID: (Human) 7314
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