|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human uroplakin III.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is bladder carcinoma.
Uroplakins are specific differentiation products of terminally differentiated superficial urothelial cells. Uroplakin III is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: UP3A; UPIII; UPIIIA; UPK3; uroplakin 3; Uroplakin III; Uroplakin-3a
Gene Aliases: UP3A; UPIII; UPIIIA; UPK3; UPK3A
UniProt ID: (Human) O75631
Entrez Gene ID: (Human) 7380
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