|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||20 µg/mL|
|Immunofluorescence (IF)||20 µg/mL|
|Western Blot (WB)||0.25 µg/mL|
|Western Blot (WB)||See 15 publications below|
|Miscellaneous PubMed (MISC)||See 16 publications below|
|Immunocytochemistry (ICC)||See 7 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Yeast|
|Published species reactivity||Yeast|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified S. cerevisiae vacuole membranes|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C|
This clone recognizes the 60 kDa subunit 1 (B-subunit, the product of the VMA2 or VAT2 gene) of S. cerevisiae. By Western blot, this can be used to identify organelles during subcellular fractionation of yeast membranes.
Subunit B (~57kDa) is a non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles. This polypeptide is associated with the cytoplasmic face of the vacuolar membrane when the V-ATPase complex is fully assembled.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: V-ATPase 57 kDa subunit; V-ATPase subunit B; V-type proton ATPase subunit B; Vacuolar proton pump subunit B; Vma2p
Gene Aliases: VAT2; VMA2; YBR1002; YBR127C
UniProt ID: (Baker's yeast) P16140
Entrez Gene ID: (Baker's yeast) 852424
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