|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||1:500 - 1:2000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000 - 1:3000|
|Immunoprecipitation (IP)||See 1 publications below|
|Western Blot (WB)||See 5 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15253 recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins.
MA5-15253 has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications.
MA5-15253 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: V5 Epitope Tag
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