|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Western Blot (WB)||0.5-3 µg/ml|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant rat VAMP-4 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-768 detects VAMP-4 from human and mouse samples.
PA1-768 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~18 kDa protein representing VAMP-4 from PC3 cell extract. Immunofluorescent staining of VAMP-4 in CV-1 cells using PA1-768 results in a staining pattern consistent with VAMP-4 staining.
Immunofluorescence staining with this antibody requires saponin permeabilization.
The PA1-768 antigen is recombinant rat VAMP-4.
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer and quote;s disease, VAMP levels of all isoforms appear to be significantly lowered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: VAMP-4; Vesicle Associated Membrane Protein 4; Vesicle-associated membrane protein 4
Gene Aliases: D1Ertd147e; VAMP-4; VAMP24; VAMP4
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