|Flow Cytometry (Flow)||1/400|
|Immunohistochemistry (Frozen) (IHC (F))||1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2,000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 8 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Yeast , Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-004 detects VCP protein in human, mouse, and rat samples.
MA3-004 has successfully been used in Western blot immunoprecipitation, and immunohistochemical procedures. By Western blot, this antibody detects an ~97 kDa protein representing VCP from total lysate of cultured human B cells.
The MA3-004 immunogen is a synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP. This peptide (Cat. # PEP-239) is available for use in neutralization and control experiments.
Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins.
VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 15S Mg(2+)-ATPase p97 subunit; AAA ATPase p97; epididymis luminal protein 220; epididymis secretory protein Li 70; homolog of yeast cdc48; Inv protein; TER ATPase; Transitional endoplasmic reticulum ATPase; Valosin-Containing Protein; VCP; yeast Cdc48p homolog
Gene Aliases: 3110001E05; ALS14; CDC48; CMT2Y; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; p97/VCP; TERA; VCP
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