|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human VEGF|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is angiosarcoma or placenta tissue.
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. This antibody reacts with the 165, 189 and 121 amino acid splice variants of VEGF of human, to a lesser extent, mouse and rat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: vascular endothelial growth factor; Vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; VEGF-A; VPF
Gene Aliases: MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) P15692
Entrez Gene ID: (Human) 7422
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