|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-3 µg/mL|
|Western Blot (WB)||See 2 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Cat , Human|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11690 targets Vinculin in IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-11690 immunogen is semi-purified vinculin.
Vinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and alpha actinin, which in turn binds to actin. The binding of fibronectin is also indirect where vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor which itself is the ligand for fibronectin. Vinculin is widely distributed in tissue and is expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: epididymis luminal protein 114; Metavinculin; MV; Vinculin
Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL
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