|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to residues in human WNK2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 243 kDa.
Purity is >95% by SDS-PAGE.
The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250-300 amino acids organized in two domains. WNK, for "with no lysine (K)", kinases are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydrophobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1. The human WNK1 gene encodes a 2,382 amino acid protein that is primarily expressed in heart, kidney, muscle and distal nephron. The human WNK3 gene encodes a protein that is primarily expressed in brain; the human WNK4 gene encodes a 1,243 amino acid protein that is expressed in kidney. Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption and impaired K+ and H+ excretion.
Protein Aliases: Antigen NY-CO-43; KIAA1760; mitogen-activated protein kinase kinase kinase; P/OKcl.13; PRKWNK2; Protein kinase lysine-deficient 2; Protein kinase with no lysine 2; protein kinase, lysine deficient 2; SDCCAG43; Serine/threonine-protein kinase WNK2; Serologically defined colon cancer antigen 43
Gene Aliases: 1810073P09Rik; AW122246; ESTM15; KIAA1760; mKIAA1760; NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43; WNK2; X83337