|Immunocytochemistry (ICC)||10 ug/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunohistochemistry (IHC)||2 µg/ml|
|Western Blot (WB)||0.5-2 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~32kDa. A suggested positive control is human spleen tissue lysate.
PA5-20038 can be used with blocking peptide PEP-0157.
XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BIRC4 binding protein; BIRC4-binding protein; F-box protein 39; novel TRAF-type zinc finger protein; XIAP-associated factor 1
Gene Aliases: BIRC4BP; Fbox39; HSXIAPAF1; XAF1; XIAPAF1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.