|Flow Cytometry (Flow)||2-3 µg/ml|
|Immunohistochemistry (IHC)||5 µ/gml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acid 1-50 of the human XCT protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody is not recommended for Immunoprecipitation.
Permeablization is recommended prior to performing Flow analysis.
Suggested positive control: antigen standard for SLC7A11 (transient overexpression lysate), human stomach protein or stomach tissue lysate.
Mammalian amino acid transport systems consist of a large variety of transporters. The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc.
In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Amino acid transport system xc-; Amino acid transport system xc-xCT antibody; Calcium channel blocker resistance protein CCBR1; Calcium channel blocker resistance protein CCBR1 antibody.; cysteine/glutamate transporter; Cysteine/glutamate transporter antibody; Cystine/glutamate transporter; sodium independent anionic amino acid transport system; solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11; solute carrier family 7 (cationic amino acid transporter, y+ system), member 11; Solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; xCT
Gene Aliases: 9930009M05Rik; AI451155; CCBR1; SLC7A11; sut; xCT
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.