|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||His-tagged human XRCC3, over-expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody does not react with mouse samples.
The target sequence has 100% sequence homology with non-human primate and 93% sequence homology with sheep, rat, and bovine.
Suggested positive control: antigen standard for XRCC3 (transient overexpression lysate).
Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. XRCC3 (x-ray cross complementing) was first identified as a human gene complementing the DNA damage sensitivity, chromosonal instability and impaired growth of the mutant hamster cell line irs1SF. This fact coupled with XRCC3 being a member of the RAD51 gene family have implied a role in the repair of DNA damage by homologous recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA repair protein XRCC3; X ray repair complementing defective repair in Chinese hamster cells 3; X ray repair cross complementing protein 3; X-ray repair complementing defective repair in Chinese hamster cells 3; X-ray repair cross-complementing protein 3
Gene Aliases: CMM6; XRCC3
UniProt ID: (Human) O43542
Entrez Gene ID: (Human) 7517
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