|Western Blot (WB)||1-2 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 17 amino acid peptide from near the amino terminus of human ZBED3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
Despite its predicted molecular weight, ZBED3 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is A-20 cell lysate.
PA5-20767 can be used with blocking peptide PEP-0881.
Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.
Protein Aliases: 2610005H11Rik; Axin-interacting protein; Zinc finger BED domain-containing protein 3; zinc finger, BED domain containing 3; zinc finger, BED-type containing 3
Gene Aliases: 0610037K01Rik; 2610005H11Rik; AU018975; AU024588; ZBED3