|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid peptide near the center of human ZEB1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is Hela cell lysate.
PA5-20979 can be used with blocking peptide PEP-1093.
ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha(4)-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.
Protein Aliases: delta-crystallin enhancer binding factor 1; Negative regulator of IL2; NIL-2-A zinc finger protein; posterior polymorphous corneal dystrophy 3; RP11-472N13.4, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein
Gene Aliases: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZEB1; ZFHEP; ZFHX1A
UniProt ID: (Human) P37275
Entrez Gene ID: (Human) 6935