|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Immunocytochemistry (ICC)||See 108 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 40 publications below|
|Western Blot (WB)||See 109 publications below|
|Immunohistochemistry (IHC)||See 60 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 19 publications below|
|Miscellaneous PubMed (MISC)||See 52 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunofluorescence (IF)||See 42 publications below|
|Immunoprecipitation (IP)||See 12 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 3 publications below|
|ELISA (ELISA)||See 2 publications below|
|Tested Species reactivity||Dog, Guinea pig, Human, Mouse, Rat|
|Published species reactivity||Tag , Baboon , Rat , Pig , Non-human primate , Virus , Insect , Bovine , Sheep , Cat , Zebrafish , Plant , Human , Mouse , , Horse , Xenopus , Rhesus monkey , Guinea pig , Dog , Rabbit C. elegans Fruit fly|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 69 kD fusion protein(1) corresponding to amino acids 463-1109 of human ZO-1 cDNA.(2) This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
61-7300 has been successfully used in Western blot, Immunoprecipitation, Immunofluorescence, ELISA and Immunohistochemistry.
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
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