|Immunohistochemistry (Frozen) (IHC (F))||2-3µg/ml|
|Western Blot (WB)||3-4µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Dog , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of mouse ZONAB (ZO-1 associated nucleic acid-binding protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody reacts with an internal region of the ZONAB protein. On Western blots, it identifies a major band at ~65 kDa and a minor band at ~54 kDa. The antibody is directed towards a region of ZONAB common to both the long and the alternatively-spliced short form. During development, reactivity was confirmed using mouse testis tissue homogenates by Western blotting and labeling of mouse testis tissue using immunofluorescence. All signals were absent in knockout mice, further confirming the reactivity with ZONAB.
ZONAB (ZO-1 associated nucleic acid-binding) is a Y-box transcription factor whose nuclear localization and transcriptional activity is regulated by the tight junction-associated candidate tumor suppressor ZO-1. Two ZONAB isoforms are known, a long isoform (ZONAB B) and an alternatively spliced shorter isoform (ZONAB A) that lacks 68 amino acids. ZONAB regulates the expression of the tyrosine kinase receptor HER2, and appears to act in combination with ZO-1 to regulate HER2 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cold shock domain protein A; Cold shock domain-containing protein A; DNA-binding protein A; Muscle Y-box protein YB2; RYB-A; Y-box protein 3; Y-box-binding protein 3; Y-box-binding protein A; ZO-1 associated nucleic acid binding protein; ZONAB A; ZONAB B; zonula occludens 1 associated nucleic acid binding protein
Gene Aliases: Csda; Dbpa; Dpba; MSY3; MSY4; oxyR; Yb2; Ybx3
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.