|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1µg per 10^6 cells|
|Immunofluorescence (IF)||4 ug/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||4 µg/ml|
|Western Blot (WB)||2 ug/mL|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-800 detects the alpha Actinin 4 in human and mouse cells.
MA1-800 has been successfully used in Western blot, and immunofluorescence procedures. MA1-800 detects alpha Actinin 4 which has a predicted molecular weight of approximately 105 kDa. Under reducing conditions, MA1-800 detects a monomer of alpha Actinin 4 at approximately 52.5 kDa.
The MA1-800 immunogen is a synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actin 4.
alpha-Actinin 4, a member of the alpha-Actinin family of cytoskeletal proteins, functions as an actin-filament crosslinking protein. It has been shown to play a role in cell motility, endocytosis, and glomerular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actinin-Alpha-4; Actn4; Alpha-actinin-4; F-actin cross-linking protein; F-Actin cross-linking protein.; focal segmental glomerulosclerosis 1; Non-muscle alpha-actinin 4
Gene Aliases: ACTININ-4; ACTN4; C77391; FSGS; FSGS1
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