|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 698 and 911 of Human alpha Actinin 4|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27475 targets alpha Actinin 4 in IF/ICC, IHC (P), and WB applications and shows reactivity with Human, Rat and mouse samples.
The PA5-27475 immunogen is recombinant fragment corresponding to a region within amino acids 698 and 911 of Human alpha Actinin 4.
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actinin alpha4 isoform; ACTININ-4; alpha actinin 4; Alpha-actinin-4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; FSGS; FSGS1; Non-muscle alpha-actinin 4
Gene Aliases: ACTININ-4; ACTN4; C77391; FSGS; FSGS1
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