|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 245 and 496 of Human alpha Adaptin 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-29528 targets alpha Adaptin 2 in WB applications and shows reactivity with mouse samples.
The PA5-29528 immunogen is recombinant fragment corresponding to a region within amino acids 245 and 496 of Human alpha Adaptin 2.
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF) receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern of expression.
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Protein Aliases: 100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; Adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; Adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex AP-2, alpha 1 subunit; ADTAB, CLAPA2, HIP-9, HIP9, HYPJ, 100 kDa coated vesicle protein C, AP-2 complex subunit alpha-2, adaptin, alpha B, alpha-adaptin C; Alpha-adaptin A; Alpha1-adaptin; AP-2 complex subunit alpha-1; Clathrin assembly protein complex 2 alpha-A large chain; Huntingtin interacting protein J, alpha2-adaptin, clathrin assembly protein complex 2 alpha-C large chain, clathrin-associated/assembly/adaptor protein, large, al; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Gene Aliases: Adtaa; Ap2a1; Clapa1
UniProt ID: (Mouse) P17426
Entrez Gene ID: (Mouse) 11771
Molecular Function: transmembrane receptor regulatory/adaptor protein
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