|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 µg/mL|
|Western Blot (WB)||See 8 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Amphibian, Bovine, Chicken, Human, Mouse, Non-human primate, Pig, Rat|
|Published species reactivity||Rat , Non-human primate , Amphibian , Bovine , Mouse Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified bovine brain adaptor complexes.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-061 detects alpha-adaptin from bovine, chicken, frog, human, monkey, mouse, pig and rat. This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.
MA3-061 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract. Immunofluorescence staining of alpha-adaptin in transfected COS cells with MA3-061A yields distinct punctate dots on the plasma membrane.
The MA3-061 antigen is purified bovine brain adaptor complexes.
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein between the clathrin lattice and the membrane.
There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF) receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern of expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; Adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; adaptor related protein complex 2, alpha 1 subunit; Adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; adaptor-related protein complex AP-2, alpha 1 subunit; Alpha-adaptin A; Alpha1-adaptin; AP-2 complex subunit alpha-1; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Gene Aliases: ADTAA; AP2-ALPHA; AP2A1; BOS_17797; CLAPA1
Molecular Function: transmembrane receptor regulatory/adaptor protein
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