|Western Blot (WB)||0.5-1.0 µg/ml|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 70-140 of the human Crystallin AB protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa.
There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex.
CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.Suggested positive control: human and mouse skeletal muscle lysate, human skeletal muscle protein, antigen standard for HSPB1 (transient overexpression lysate), mouse skeletal muscle protein.
Crystallin AB (CryAB) is a developmentally regulated small heat shock protein known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is known to be associated with a number of neurodegenerative pathologies such as Alzheimer disease amd multiple sclerosis. As well, it has been found to be expressed in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha (B)-crystallin antibody; alpha B-crystallin; Alpha crystallin B chain antibody; Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha 2; crystallin, alpha B; epididymis secretory protein Li 101; Heat shock protein beta-5; heat shock protein CryAB; heat shock protein HspB5; heat-shock 20 kD like-protein; Heat-shock protein beta-5 antibod; HspB5; P23; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component; Rosenthal fiber componet antibody
Gene Aliases: CMD1II; Crya-2; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; P23
Molecular Function: chaperone
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