|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000-1:2000|
|Tested Species reactivity||Bovine, Hamster, Human, Mouse, Pig, Rabbit, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide:R(163)EEKPAVTAAPKK(175) KLH conjugated, corresponding to human alpha B Crystallin.|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-20795 detects AlphaB Crystallin in Human, Mouse, Rat, Bovine and Porcine samples. Expected to cross react with hamster and rabbit due to sequence homology. This antibody will not cross react with beta Crystallin nor alpha A Crystallin.
PA1-20795 has been successfully used in Immunocytochemistry, Immunoprecipitation and Western Blot procedures. Detects a band ~22 kDa.
PA1-20795 immunogen corresponds to synthetic peptide:R(163)EEKPAVTAAPKK(175) KLH conjugated, corresponding to human alpha B Crystallin.
Lens proteins consist almost entirely of Crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha Crystallin is the largest of the crystallins and is composed of 2 primary gene products; alpha A and alpha B. There are at least 5 different proteins comprising the beta Crystallins. The gamma Crystallins are monomeric, but there are at least 5 gamma Crystallins identified in bovine and rat lens. Alpha Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha Crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha B Crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
Protein Aliases: alpha B-crystallin; Alpha(B)-crystallin; alpha-B-crystallin; Alpha-crystallin B chain; alpha-crystallin B chain-like protein; CRYA2; crystallin, alpha 2; crystallin, alpha B; crystallin, alpha polypeptide 2; CTPP2; epididymis secretory protein Li 101; Heat shock protein beta-5; heat shock protein CryAB; heat shock protein HspB5; heat-shock 20 kD like-protein; HSPB5; P23; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; BOS_14343; CMD1II; Crya-2; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; P23
Molecular Function: chaperone