|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
|Immunoprecipitation (IP)||See 1 publications below|
|Western Blot (WB)||See 4 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||90 kDa MBP/alpha catenin fusion protein purified on amylose column.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-2000 detects alpha catenin from human samples.
MA1-2000 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~102 kDa protein representing alpha catenin from scc-1 cell extract. In Western blot, this antibody did not cross-react with mouse samples. MA1-2000 can also be used in immunoprecipitation and immunofluorescence procedures.
The MA1-2000 immunogen is a 90 kDa MBP/alpha catenin fusion protein purified via an amylose column.
Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. Mice that have a-catenin ablated in their surface epithelium demonstrate reduced hair growth, multinucleated cells, extensive proliferation and a general cancerous phenotype.
Protein Aliases: Alpha E-catenin; alpha-E-catenin; Cadherin-associated protein; cadherin-associated protein alpha; catenin (cadherin-associated protein), alpha 1, 102kDa; Catenin alpha-1; Renal carcinoma antigen NY-REN-13
Gene Aliases: CAP102; CTNNA1; MDPT2
UniProt ID: (Human) P35221
Entrez Gene ID: (Human) 1495