|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 - 3 µg/mL|
|Tested Species reactivity||Amphibian, Bovine, Dog, Gerbil, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the residues M(426) A A L E K D Y E E V G V D S V E G E G E E E G E E(450) of human alpha Tubulin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, do not freeze|
PA1-20988 detects apha Tubulin in human, mouse, rat, Xenopus laevis, bovine, guinea pig, gerbil, and porcine samples. Expected to cross react with newt (100% identity with immunogen) due to sequence homology.
PA1-20988 has been successfully used in immunofluorescence, immunohistochemistry (FFPE), immunoprecipitation and Western blot procedures. By Western blot, PA1-20988 detects a ~55 kDa band representing alpha Tubulin. Formalin-fixed paraffin embedded tissue sections require boiling in 10 mM citrate buffer (pH 6.0) prior to immunostaining. Positive control of lung tissue slides suggested.
The PA1-20988 immunogen is a synthetic peptide corresponding to the residues M(426) A A L E K D Y E E V G V D S V E G E G E E E G E E(450) of human alpha Tubulin. This sequence is 100% conserved in newt alpha Tubulin.
Fig. 1 shows specific staining of paraffin embedded human lung sections using PA1-20988.
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha-tubulin 1; Alpha-tubulin 4; Alpha-tubulin isotype M-alpha-4; FLJ30169; H2-ALPHA; Testis-specific alpha-tubulin; TUBA1; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin H2-alpha; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a
Gene Aliases: ALS22; BOS_2117; H2-ALPHA; M[a]4; TUBA1; Tuba4; TUBA4A
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