|Flow Cytometry (Flow)||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1,000 - 1:4,000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of humun tubulin alpha|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with mouse, bovine, guinea pig, porcine, gerbil, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha-tubulin 1; Alpha-tubulin 4; Alpha-tubulin isotype M-alpha-4; FLJ30169; H2 ALPHA; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA1; Tubulin alpha; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin H2-alpha; tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a
Gene Aliases: ALS22; H2-ALPHA; M[a]4; TUBA1; Tuba4; TUBA4A
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