|Flow Cytometry (Flow)||0.01 µg/10^6 cells|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2500|
|Western Blot (WB)||0.5 µg/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Bovine, Dog, Chicken, Deer, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
PA5-19489 targets alpha Tubulin in immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with chicken, Indian muntjac, Chinese hamster, human, fruit fly, rat, African Green Monkey, and mouse samples. Perform heat mediated antigen retrieval via the pressure cooker method before beginning immunohistochemistry staining protocol.
The PA5-19489 immunogen is synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
PA5-19489 detects alpha Tubulin which has a predicted molecular weight of approximately 50 kDa.
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 4; alpha-Tubulin 84B; alpha-Tubulin at 84B; Alpha-tubulin isotype M-alpha-4; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[] tubulin; alpha[]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; CG1913-PA; FLJ30169, H2-ALPHA, TUBA1, alpha-tubulin 1; Testis-specific alpha-tubulin; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin H2-alpha; tubulin(alpha1); tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a; Tubulin-alpha1; tubulin1alpha; tubulin84B
Gene Aliases: 1t; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; BEST:LD32507; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; H2-ALPHA; l(3)84Bd; l(3)g3; M[a]4; ms(3)nc33; nc33; T; Talpha1; TBA1_DROME; Tub; TUBA1; Tuba4; TUBA4A; Tuba84B; Tubalpha1; tubulin
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